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  • Two-step RT-qPCR analysis of expression of 7 drought-related genes in tomato (Lycopersicon esculentum Mill.)

    Ivana Petrović

    Chapter from the book: Vucelić-Radović, B et al. 2019. Application of Molecular Methods and Raman Microscopy/Spectroscopy in Agricultural Sciences and Food Technology.

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    The identification and characterization of genes induced under drought stress is a common approach to elucidate the molecular mechanisms of drought stress tolerance in plants. Examination of gene expression using quantitative PCR (qPCR) in combination with Reverse Transcription (RT) in plant responses to drought stress can provide valuable information for stress-tolerance improvement. The purpose of this manuscript is to describe procedure for two step RT-qPCR analysis of gene expression in tomato leaves, under control conditions and under drought stress. Described protocol can be adjusted and used for gene expression analysis of different plant species.

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    How to cite this chapter
    Petrović, I. 2019. Two-step RT-qPCR analysis of expression of 7 drought-related genes in tomato (Lycopersicon esculentum Mill.). In: Vucelić-Radović, B et al, Application of Molecular Methods and Raman Microscopy/Spectroscopy in Agricultural Sciences and Food Technology. London: Ubiquity Press. DOI: https://doi.org/10.5334/bbj.a
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    This is an Open Access chapter distributed under the terms of the Creative Commons Attribution 4.0 license (unless stated otherwise), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. Copyright is retained by the author(s).

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    Additional Information

    Published on July 23, 2019

    DOI
    https://doi.org/10.5334/bbj.a


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