Real-time PCR detection of quarantine plant pathogenic bacteria in potato tubers and olive plants
Chapter from the book: Vucelić-Radović, B et al. 2019. Application of Molecular Methods and Raman Microscopy/Spectroscopy in Agricultural Sciences and Food Technology.
This chapter focuses on molecular detection of quarantine plant pathogenic bacteria associated with potato and olive plants. A real-time PCR for detection of two bacteria of potato in Europe: Ralstonia solanacearum race 3 and Clavibacter michiganensis subsp. sepedonicus, is described. This method allows the simultaneous detection of both species in a single PCR reaction with an internal control from potato. Described protocol is sensitive and specific and can be used in large scale screening tests. Quarantine pest Xylella fastidiosa is recently detected in Europe for the first time. Considering the importance of early detection, procedure for real-time PCR detection of Xylella fastidiosa in olive plant tissue is described.